Now Playing Tracks

Here are some tips, provided by Applied Biosystems, for Polymerase Chain Reaction (PCR) success!

PCR is an invaluable tool used by molecular biologists in order to amplify a small amount of DNA into an infinitely larger amount. PCR is often used in foresenic laboratories when a small droplet of blood is found at a crime scene and it needs to be amplified. It is also used in gene cloning and you can even add additional bases to the end of your primers for ligase reactions. You can even use real time, or quantitative PCR to measure gene expression!

Additional tips from molecularbiologistproblems:

1. Use gloves at all times! The deoxyribonucleic acidases (DNAases) on your hands will sabotage your entire reaction.

2. Triple check your primer sequences before ordering them! Have a colleague confirm your sequence.

3. More to come…

72 notes

  1. forensicgenetics reblogged this from molecularbiologistproblems
  2. blackbird315 reblogged this from molecularbiologistproblems
  3. under-lock-and-keyy reblogged this from molecularbiologistproblems
  4. sunlightandmoonshine reblogged this from molecularbiologistproblems and added:
    I remember my grad student telling me this when he made me make primers and order them. So handy~
  5. thatonewiseguy reblogged this from molecularbiologistproblems
  6. curiousity1killed2the3cat reblogged this from molecularbiologistproblems
  7. oxidoreductase reblogged this from molecularbiologistproblems
  8. planethodgepodge reblogged this from molecularbiologistproblems
  9. ryanbroadley reblogged this from thejunglenook
  10. thejunglenook reblogged this from eternalacademic and added:
    I’m positive we had a sacrificial altar in the back of the lab for prayers to the PCR gods.
  11. eternalacademic reblogged this from parttimebiologistfulltimeninja and added:
    Also pray to all known deities and you might want to sacrifice some animals as well.
  12. parttimebiologistfulltimeninja reblogged this from molecularbiologistproblems
To Tumblr, Love Pixel Union