Here are some tips, provided by Applied Biosystems, for Polymerase Chain Reaction (PCR) success!
PCR is an invaluable tool used by molecular biologists in order to amplify a small amount of DNA into an infinitely larger amount. PCR is often used in foresenic laboratories when a small droplet of blood is found at a crime scene and it needs to be amplified. It is also used in gene cloning and you can even add additional bases to the end of your primers for ligase reactions. You can even use real time, or quantitative PCR to measure gene expression!
Additional tips from molecularbiologistproblems:
1. Use gloves at all times! The deoxyribonucleic acidases (DNAases) on your hands will sabotage your entire reaction.
2. Triple check your primer sequences before ordering them! Have a colleague confirm your sequence.
3. More to come…